1. Serial Dilution Procedure

Serial Dilution Procedure

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1. Continue aliquoting 10mL of subsequent dilutions into 90ml filled PBS diluent vessels until the desired concentration of test sample is obtained. Each succeeding dilution increases by a factor of 10. A separate sterile pipet should be used with each dilution. 1:100 Serial Dilutions 1a.
2. Serological tests may be performed for diagnostic purposes when an infection is suspected, in rheumatic illnesses, and in many other situations, such as checking an individual's blood type. Serology blood tests help to diagnose patients with certain immune deficiencies associated with the lack of antibodies, such as X-linked agammaglobulinemia.

By performing two-fold serial dilutions on the serum prior to testing, the concentration of the serum antibodies can be expressed as an HI titre to the log base 2. Standardization of the HI test. It is important that there is correlation between the results of tests carried out by different technicians and in different laboratories.